service - Cryo-EM SPA
B A C KCryo-EM Services for Protein Structure Determination
As one technique of Cryo-EM, Single particle analysis (SPA) is used to determine structures of biomacromolecules frozen in vitreous ice and enables researchers to determine the protein structures in near-native states.Now SPA is becoming important in structural studies of medically relevant targets.
Cryo-EM SPA Workflow
Why do you need Cryo-EM SPA Services?
● Maintain the sample in its near-native state
● Require a only a small amount of protein
● Capture flexible conformations
● Determine heterogenous protein complex structures
Why choose ShuimuBio for Cryo-EM SPA?
● ShuimuBio is the first and largest commercial cryo-EM platform in the Asia-Pacific.
● We are equipped with the highest specs microscopes and advanced IT infrastructure to ensure the high-throughput and high-quality of our projects.
● Our team is a world leader in the research and application of cryo-electron microscopy technology, more and more complex protein structures are being resolved.
● 300+ Cryo-EM projects，including apo-protein, small molecule and protein complex, antigen and antibody complex were supported, 60+ proteins were determined at resolution better than 3.5 Å, the best resolution reaches 1.8 Å and the smallest protein being constructed is about 51KD in our platform.
About the samples
Protein （50-100μl，≥2mg/mL，Purity>95%）:
SDS-PAGE to check no polluted or degraded protein bands. Make sure it is the target protein (mass spectrometry identification, etc.)
Buffer （50-100ml):
Do not contain organic substances such as polysaccharides, DMSO, glycerol. Salt concentration should below 300mM.
Homogeneity:
Gel filtration chromatography or native page (or other methods) is necessary to ensure sample homogeneity. Do not overconcentrate sample to avoid protein aggregation, etc.
Storage (10-20μl/tube):
Samples should be divided into aliquots, flash frozen and stored in liquid nitrogen. Avoid repeated freezing and thawing.